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Literature summary extracted from

  • Ball, P.; Halliwell, J.; Anderson, S.; Gwenin, V.; Gwenin, C.
    Evaluation of two xenobiotic reductases from Pseudomonas putida for their suitability for magnetic nanoparticle-directed enzyme prodrug therapy as a novel approach to cancer treatment (2020), MicrobiologyOpen, 9, e1110 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.B1 expressed in Escherichia coli Rosetta pLysS cells Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.B1 0.457
-
 CB1954 genetically modified protein XenB-cys, at pH 7.2 temperature not specified in the publication Pseudomonas putida
1.7.1.B1 1.69
-
 CB1954 genetically modified protein XenB-his, at pH 7.2 temperature not specified in the publication Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.B1 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.B1 CB1954 + NADPH + H+
-
 Pseudomonas putida ? + hydroxylamine + NADP+
-
 ?
1.7.1.B1 additional information the enzyme shows no reactivity with CB1954 Pseudomonas putida ?
-
-

Subunits

EC Number Subunits Comment Organism
1.7.1.B1 homodimer 2 * 39000, SDS-PAGE Pseudomonas putida
1.7.1.B1 homodimer 2 * 40000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.7.1.B1 XenA
-
 Pseudomonas putida
1.7.1.B1 XenB
-
 Pseudomonas putida
1.7.1.B1 xenobiotic reductase A
-
 Pseudomonas putida
1.7.1.B1 xenobiotic reductase B
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.B1 1.8
-
 CB1954 genetically modified protein XenB-cys, at pH 7.2 temperature not specified in the publication Pseudomonas putida
1.7.1.B1 8.2
-
 CB1954 genetically modified protein XenB-his, at pH 7.2 temperature not specified in the publication Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.1.B1 3.9
-
 CB1954 genetically modified protein XenB-cys, at pH 7.2 temperature not specified in the publication Pseudomonas putida
1.7.1.B1 4.9
-
 CB1954 genetically modified protein XenB-his, at pH 7.2 temperature not specified in the publication Pseudomonas putida